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Image Search Results
Journal: Artificial cells, nanomedicine, and biotechnology
Article Title: Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21.
doi: 10.1080/21691401.2020.1725533
Figure Lengend Snippet: Figure 6. Silence of MEG3 activates PI3K/AKT pathway while deactivates NF-jB pathway via targeting miR-21. PC12 cells were co-transfected with si-MEG3/si-NC and miR-21 inhibitor/NC inhibitor, and then incubated in hypoxia for 24 h. The cells without transfection in normoxic condition served as control (CTRL). The expres- sion of proteins involved in (A,B) PI3K/AKT and (C,D) NF-jB pathways was tested by western blot. n ¼ 3.p < .05 represents a comparison of marked groups.
Article Snippet: Proteins were carried out by using primary antibodies specific for CyclinD1 (orb77046), p53 (orb178524), p21 (orb38089), Bcl-2 (orb378569), Bax (orb378566), pro-caspase-3 (orb13278), cleaved-caspase-3 (orb310032), PTEN (orb39110), PI3K (orb395443),
Techniques: Transfection, Incubation, Control, Western Blot, Comparison
Journal: Cellular and molecular biology (Noisy-le-Grand, France)
Article Title: Pro-inflammatory GPR75 and anti-apoptotic phospholipase signaling pathways contribute to the ameliorating effect of soluble epoxide hydrolase inhibition on chronic experimental autoimmune encephalomyelitis in mice.
doi: 10.14715/cmb/2023.69.10.2
Figure Lengend Snippet: Figure 3. TPPU increased the PLC β4, PI3K p85α, Akt1, MEK1/2, ERK1/2, CREB1, and Bcl-2 expression and/or activity induced by MOG35-55 peptide/PT in the (a, b, c, d, e, f, and g) brain and (h, i, j, k, l, m, and n) spinal cord of PBS-, DMSO-, or TPPU-injected mice. The expression of unphosphorylated and phosphorylated pro teins for PLC β4, PI3K p85α, Akt1, MEK1/2, ERK1/2, CREB1, and Bcl-2 in tissues was measured by immunoblotting technique. Data are expressed as means ± SEM of 3-4 animals. + P<0.05 versus PBS- injected mice (EAE control group); # P<0.05 versus DMSO-injected mice (vehicle control group).
Article Snippet: Nitrocellulose membranes containing transferred proteins from the gel were blocked with nonfat dry milk in Tris-buffered saline and then incubated with specific antibodies against (1) sEH (13560; Cayman), (2) GPR75 (GTX55193; GeneTex, Hsinchu City, Taiwan), (3) Gαq/11 (sc-515689; Santa Cruz Biotechnology, Santa Cruz, CA, USA), (4) c-Jun (sc-1694; Santa Cruz), (5) phosphorylated c-Jun (p-c-Jun) (sc-16312; Santa Cruz), (6) PLC β4 (sc-16631; Santa Cruz); (7) PI3K p85α (OAAF05915; Aviva Systems Biology, San Diego, CA, USA), (8)
Techniques: Expressing, Activity Assay, Injection, Western Blot, Control
Journal: OncoTargets and Therapy
Article Title: Exosome-Delivered EGFR Induced by Acidic Bile Salts Regulates Macrophage M2 Polarization to Promote Esophageal Adenocarcinoma Cell Proliferation
doi: 10.2147/OTT.S437560
Figure Lengend Snippet: Discription of Antibodies Used in the Study
Article Snippet: p-PI3K ,
Techniques: Western Blot, Immunohistochemistry, Control